Genorise Plasmid DNA Extraction Mini Kit 200
$395.00 – $887.00
Genorise Plasmid DNA Extraction Mini Kit uses DNA binding column to isolate plasmid DNA from 1-5 ml bacterial cells. The kit can significantly improve quality and quantity of DNA and is much more cost-effective than similar products. This kit will guarantee the quality and quantity of your DNA isolates. Genorise Plasmid DNA Extraction Mini Kits: High performance, reliable, cost-effective, your ideal choice.
- Description
- Product Citations
- Reviews (0)
Description
Features
• Rapid purification of high-quality and ready-to-use DNA.
• No organic extraction.
• Consistent, high yields.
• Complete removal of contaminants and inhibitors for reliable results.
Principle
Bacteria are lysed to solubilize the DNA contents and then proteins are removed by precipitation and DNA-containing supernatant is loaded to DNA binding column and contaminants are then washed away and finally pure DNA is eluted.
Procedures
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- Add 500 µl Buffer GB to DNA binding column with collection tube, centrifuge 13,000 x g for 1 min and discard the flowthrough.
- Centrifuge 1-5ml bacterial culture at 13,000 x g for 1 min and discard supernatant. Pipet 250 µl Buffer GS into pellet and suspend bacterial cells and transfer to a 1.5 ml microcentrifuge tube.
- Add 250 µl of Buffer GL and mix thoroughly by inverting the tube until solution becomes clear. Cell lysis should be done in 5 min.
- Add 350 µl Buffer GP and mix thoroughly by inverting the tube for 4-6 times. Centrifuge at 16, 000 x g for 5 min.
- Pipet the supernatant onto the column with collection tube and centrifuge at full speed (20,000 x g) for 1 min. If samples remain in the column, repeat centrifugation to completely remove the remaining samples. Discard the flow-through andbut keep collection tube.
- Add 500 µl Buffer GW1 to the column with collection tube and add 500 µl Buffer GW1. Centrifuge at 6000 x g for 1 min. Discard the flow-through and keep the collection tube.
- Add 500 µl Buffer GW2. Centrifuge at full speed (20, 000 x g) for 3 min. Discard the flow-through and keep the collection tube.
- Centrifuge at full speed for 1 min. Discard the flow-through and collection tube.
- Place the mini column in a new 1.5 ml microcentrifuge tube and add 50-100 µl Buffer GE and incubate for 2 min. Centrifuge at 6000 x g for 1 min to elute DNA.
Product Citations
1. Chen J-W et al. (2010) Identification of racehorse and sample contamination by novel 24-plex STR system. Forensic Science International: Genetics 4:158-167. Impact factor: 3.940.
Products used and cited: Genorise Hair and Urine DNA Extraction Kits. Article
2. Chen J-W et al. (2014) Identification of sample donor by 24-plex short tandem repeat in a post-race equine plasma containing dexamethasone. SpringerPlus 3:94. Article
Products used and cited: Genorise Hair/blood/plasma/urine DNA Extraction kits.
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