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Genorise Blood DNA Extraction Mini Kit

Placeholder Genorise Blood DNA Extraction Mini Kit
Placeholder Genorise Blood DNA Extraction Kit-200
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$517.00 – $1,165.00

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Genorise Blood DNA Extraction Mini Kit uses DNA binding column to isolate total DNA including nucleic and mitochondrial DNA from 0.2 ml blood. The kit can significantly improve quality and quantity of DNA and is much more cost-effective than similar products. This kit will guarantee the quality and quantity of your DNA isolates. Genorise Blood DNA Extraction Mini Kits: High performance, reliable, cost-effective, your ideal choice.

CAT: GR101075 Category: Tag:
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Description

Features
• Rapid purification of high-quality and ready-to-use DNA.
• No organic extraction.
• Consistent, high yields.
• Complete removal of contaminants and inhibitors for reliable results.

Principle
Whole blood are lysed to solubilize the DNA contents and then proteins are removed by precipitation and DNA-containing supernatant is loaded to DNA binding column and contaminants are then washed away and finally pure DNA is eluted.

Procedures

  1. Pipet 200 µl Solution 1 into a 1.5 ml microcentrifuge tube. Add 200 µl whole blood and mix thoroughly by vortexing.
  2. Incubate at 56 ºC for 10 min.
  3. Add 130 µl Solution 2 and mix thoroughly by vortexing. Centrifuge at 16, 000 x g for 5 min.
  4. Place the mini column in a collection tube and pipet the supernatant onto the column and centrifuge at full speed (20,000 x g) for 1 min. If samples remain in the column, repeat centrifugation to completely remove the remaining samples. Discard the flow-through but keep collection tube.
  5. Place the column in a new collection tube and add 500 µl Solution 3. Centrifuge at 6000 x g for 1 min. Discard the flow-through and keep the collection tube.
  6. Add 500 µl solution 4. Centrifuge at full speed (20, 000 x g) for 3 min. Discard the flow-through and keep the collection tube.
  7. Centrifuge at full speed for 1 min. Discard the flow-through and collection tube.
  8. Place the mini column in a new 1.5 ml microcentrifuge tube and add 100-200 µl Solution 5 and incubate for 1 min. Centrifuge at 6000 x g for 1 min to elute DNA.

 

Product Citations

1. Chen J-W et al. (2010) Identification of racehorse and sample contamination by novel 24-plex STR system. Forensic Science International: Genetics 4:158-167. Impact factor: 3.940.
Products used and cited: Genorise Hair and Urine DNA Extraction Kits. Article
2. Chen J-W et al. (2014) Identification of sample donor by 24-plex short tandem repeat in a post-race equine plasma containing dexamethasone. SpringerPlus 3:94.  Article
Products used and cited: Genorise Hair/blood/plasma/urine DNA Extraction kits.

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