Nori Monkey IL-8 ELISA Kit - Image 1
Nori Monkey IL-8 ELISA Kit - Image 2

Nori Monkey IL-8 ELISA Kit

Nori Feline IL-8 ELISA Kit
Nori Guinea Pig IL-8 ELISA Kit
0 out of 5
( There are no reviews yet. )

Price range: $508.00 through $916.00

DataSheet   

This ELISA kit is for quantification of IL-8 in monkey. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-8 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for IL-8: Interleukin 8, CXCL8, neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI)

This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR169007 Categories: , Tags: , ,

Description

Nori Monkey IL-8 ELISA Kit Summary

Alternative names for IL-8: Interleukin 8, CXCL8, neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI)

Alternative names for monkey: Rhesus macaque, Cynomolgus macaque, Baboons, squirrel monkey, common marmoset, chimpanzee, capuchin monkey, spider monkey, howler monkey, non-human primate

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number 
P61274
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity 3 pg/mL
Detection Range 15.63-1000 pg/mL
Specificity Monkey IL-8
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

 

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background:

IL-8 was renamed CXCL8 by the Chemokine Nomenclature Subcommittee of the International Union of Immunological Societies (1), although its approved HUGO gene symbol remains IL8. CXCL8 was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL1, TNF, LPS, and viruses. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other proinflammatory effects. CXCL 8 is also a potent angiogenic factor. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a comitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells.  It is a key parameter in localized inflammation (2). It has been cited as a proinflammatory mediator in gingivitis and psoriasis (3).

References

  1. Bacon K, et al (October 2002). J. Interferon Cytokine Res. 22 (10): 1067–8.
  2. Vlahopoulos S, et al. (September 1999).  Blood 94 (6): 1878–89.
  3. Utgaard JO, et al (November 1998). Exp. Med. 188 (9): 1751–6.

 

Product Citations

  1. Jones SAL (2015) D15EQ-018 Evaluation of misoprostol as a anti-inflammatory drug in horses. North Carolina State University, usda.gov, accession # 1005887, project # NCV-VMAH-59438. Product cited: NoriÃ’ Equine IL-8 ELISA kit, Cat. # GR106476
  2. Von Antonella Victoria Block (2015) Studie Zur wiederkehrenden atemwegsobstruktium des pferdes (RAO): potentieller pathophysiologischer stellenwert von vas kularem endothelialem wachstumsfaktor (VEGF), tumornekrosefaktor-alpha (TNF-α) und vasoaktivem intestinalem peptid (VIP). Page 23. Products used and cited: Nori Equine IL-4, IL-6, IL-8, TNF-α and VEGF ELISA kits.
  3. Bharath LP et al. (2014) Imapirment of autophagy in endothelial cells prevents shear-stress-induced increase in nitric oxide bioavailability. Canadian J Physiology and Pharmacology. 92(7):605-612. Impact factor: 1.822. Products used and cited: Nori mouse IL-8 ELISA kit.
  4. Rojas M et al. (2014) Human adult bone marrow-derived stem cells decrease severity of LPS-induced acute respiratory distress syndrome in sheep. Stem Cell Res Ther 5(2):42. Impact factor: 4.731. Products used and cited: Nori Sheep IL-8 and IL-6 ELISA kits.
  5. Rajput IR et al. (2017) Saccharomyces boulardii and Bacillus subtilis B10 modulate TLRs and cytokines expression patterns in jejunum and ileum of broilers. PLOS ONE 12(3):e0173917. Impact factor: 2.806. Products used and cited: Nori Chicken IL-12, IL-17, IL-4, IFNg and IL-8 ELISA Kits.
  6. Wesseley-Szponder J et al. (2014) Crosstalk between adiponectin and cytokines (IL-6 and IL-8) during transportation stress in horses. Med Weter 70(9): 546-9. Impact factor: 0.203. Products used and cited: Nori Equine IL-6 and IL-8 ELISA Kits.
  7. Po E et al. (2016) The effects of equine plasma transfusion on markers of inflammation in healthy neonatal foals. Master of Science Thesis, University of Illinois at Urbana-Champaign. Products used and cited: Nori Equine IL-8 ELISA Kit.
  8. Coppelman ZB (2017) The use of biomarkers to determine the severity osteoarthritis in the tarsus of an older horse population. Master of Science Thesis, University of Minnesota. Products used and cited: Nori Equine IL-1β, IL-6, IL-8, IL-10 and TNFα ELISA Kits.
  9. Tabynov K et al. (2014) Safety and immunogenicity of a novel cold-adapted modified-live equine influenza virus vaccine. Australian Vet J 92(11):450-7. Impact factor: 0.887. Products used and cited: Nori Equine IL-1β, IL-6, IL-8, IL-10 and TNFα ELISA Kits.
  10. Sureshkumar S et al. (2019) Oral administration of lactobacillus reuteri expressing a 3D8 single-chain variable fragment (scFv) enhances chicken growth and conserves immune homeostasis. 3 Biotech 9:282. Impact factor: 1.497. Products used and cited: Nori Chicken IL-1β, IL-4, IL-6, IL-8, IFNγ, IGF1, and TNFα ELISA Kits.
  11. Sureshkumar S et al. (2020) Administration of L Salivarius expressing 3D8 scFv as a feed additive improved the growth performance, immune homeostasis, and gut microbiota of chickens. Animal Sc J DOI:10.1111/asj.13399. Impact factor: 1.714. Products and cited: Nori Chicken IL-1 beta, IL-6, IL-8, IL-4, IFN gamma, IGF1 and TNF alpha ELISA Kits (GR106294, GR106334, GR114139, GR106340, GR114082, GR106410, GR114119).
  12. Sureshkumar S. et al. (2020) Inclusion of Lactobacsllus salivarius strain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken. Archives of Microbiologyorg/10.1007/s00203-020-02088-3. Impact factor: 1.607. Products used and cited: Nori Chicken IL-4, IL-6, IL-8, IL-1b, TNFa, IFNg and IGF1 ELISA Kits.
  13. Vallejo-Timaran DA et al. (2021) Pre- and post-paratam concentrations of interleukin 1α, interleukin 8 and α1-acid glycoprotein in vaginal fornix and endometrium of dairy cows with clinical cervicitis. Frontiers in Vet Sci 7,605773. Impact factor: 6.34. Products used and cited: Nori Bovine IL-1α, IL-8 and AGP ELISA Kits.
  14. Veerasammy BAM (2021) Equine intestinal cells predict clinical case outcome and the use of acellular amnion to repair injury insmall intestinal strangulation. NCSU Thesis. Products used and cited: Nori Equine IL-8 ELISA Kit
  15. Sureshkumar S et al. (2022) Preliminary Study to Investigate the Effect of Lactobacillus Reuteri Administration on Growth Performance, Immunological, Gut Microbiome and Intestinal Mucosa of Chicken. Brazilian J Poult Sci 24(4):001-006. Impact factor: 0.9. http://dx.doi.org/10.1590/1806-9061-2022-1640. Products used and cited: Nori Chicken TNFa, IL-4, IL-6, IL-8, IL-1B, IFNg, IGF1 ELISA Kit.
  16. Sureshkumar S et al. (2021) Inclusion of Lactobacillus salivariusstrain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken. Archives of Microbiology 230:847-853. Impact factor: 2.8. Products used and cited: Nori Chicken IFNg, IL-8, TNFa, IL-4 ELISA Kit.
  17. Adamic N et al. (2022) Effect of intrabronchial administration of autologous adipose‑derived mesenchymal stem cells on severe equine asthma. Stem Cell Res & Therapy 13:23. https://doi.org/10.1186/s13287-022-02704-7. Impact factor: 7.9. Products used and cited: Nori Equine IL-1B, IL-4, IL-8, IL-17, TNFa, IFNg ELISA Kit.

Reviews

There are no reviews yet.

Be the first to review “Nori Monkey IL-8 ELISA Kit”