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Nori Sheep IL-1b LISA MultiSet Kit

Nori Bovine IL-1b LISA MultiSet Kit
Nori Human H-FABP ELISA MultiSet Kit
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DataSheet   

This ELISA kit is for quantification of IL-1B in sheep. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1B present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL1B is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for IL-1B: Interleukin 1 beta, IL1B

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR106448-M Categories: , Tag:

Description

Nori Sheep IL-1b ELISA MultiSet Kit Summary

Alternative names for IL-1b: Interleukon 1 beta, IL1B

Materials provided for 10-11 of 96-well plates

Description Quantity Description Quantity Description Quantity
Capture Antibody 1 Detection Antibody 1 Standard

 

 3

 

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number P21621
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity 12 pg/mL
Detection Range 62.5-4000 pg/mL
Specificity Sheep IL-1b
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

 

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2.Plate preparation.

3. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
4. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
5. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
6. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
7. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

Interleukin-1 beta (IL-1β) also known as catabolin, is a member of the interleukin 1 cytokine family.  IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1β.  Interleukin-1 alpha and interleukin-1 beta forms IL-1 (1,2,3). IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1β gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (4).

References

  1. Auron PE, et al (1984). Proc Natl Acad Sci U S A 81 (24): 7907–11.
  2. March CJ, et al. (1985). Nature 315 (6021): 641–7.
  3. Clark BD, et al (1986). Nucleic Acids Res 14 (20): 7897–1914.
  4. Entrez Gene: IL1B interleukin 1, beta. http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3553.

Product Citations

Nori IL-1b ELISA Kit Citations

Since 2014, updated on 5-Apr-26

  1. Razavi SM (2015) Malignant ovine theileriosis: serum concentrations of some inflammatory components and adenosine deaminase activity. Comparative Clinical Pathology 24(3):639-643. Impact factor: 0.47.

Product used and cited: Nori Sheep IL-1β ELISA Kit

  1. El-Ashker M et al. (2015) Vitamin C ameliorates gentamicin-induced acute kidney injury in equines: an experimental study. Equine Vet Sci. 35:1-6. Impact factor: 0.29.

Products used and cited: Nori Equine IL-β, IL-6, IL-10 and IFNg ELISA kits.

  1. Azma F et al. (2015) A study on the status of inflammatory systems in camels naturally infected with Toxoplasma gondii. Tropical Animal Health and Production 47:909-914. Impact factor: 1.22.

Products used and cited: Nori Bovine IL-1β ELISA kit

  1. Sandersen C et al. (2015) Effect of inhaled hydrosoluble curcumin on inflammatory markers in broncho-alveolar lavage fluid of horses with LPS-induced lung neutrophilia. Multisciplinary Respiratory Medicine. 10:16. Impact factor: 1.96.

Products used and cited: Nori Equine TNF-alpha, IL-1β and IL-6 ELISA kits

  1. Kopp L et al. (2015) The effects of dental pulp derived stem cells on the interleukin response in equine osteoarthritis. The Ohio State University Honors Theses.

Products used and cited: Nori Equine IL-1β, IL-1RA, IL-6 and IL-10 ELISA kits.

  1. El-Ashker M et al. (2014) Gentamicin-induced acute kidney injury in equines is associated with marked acute phase response: an experimental study on donkey (Equus asinus). J Vet Sci Med Diagn 4:2. Impact factor: 0.65.

Products used and cited: Nori Equine IL-1β, IL-6, IFN-g, IL-10, SAA, Haptoglobin, CRP and SA ELISA kits.

  1. Kopp L et al. (2015) Synovial fluid parameters from osteoarthritic equine joints treated with intra-articulara equine stem cells or control solution. Advances in Veterinary Medicine Research, 2 April 2015, Books of Abstracts, The Ohio State University Press, p23:CR-15.

Products used and cited: Nori Equine IL-1β, IL-1RA, IL-6 and IL-10 ELISA kits.

  1. Da Silva et al. (2014) Horse seropositive for neospora spp.: Immunoglobulin, cytokines, and c-reactive protein levels. J Equine Vet Sci. 34(10):1240-143. Impact factor: 0.882.

Products used and cited: Nori Equine TNF-α, IFNg, IL-1β, IL-1RA, IL-4, IL-6 and IL-10 ELISA kits.

  1. Do Carmo GM (2015) Immunological response and markers of cell damage in seropositive horses for Toxoplasma gondii. Comparative Immunology, Microbiology and Infectious Diseases. 38:9-13. Impact factor: 1.875.

Products used and cited: Nori Equine IL-1β, IL-4, IL-6, IL-10, IFNg and TNF-α ELISA Kits

  1. Bryn DE et al. (2016) Fetal brain development involves activation of inflammatory pathways in the fetal cerebral cortex: increased tissue content of interleukin 1β. The FASEB Journal 30(1) Supplement 1247.17. Impact factor: 5.498

Product used and cited: NoriÒ Sheep IL-1β ELISA kit

  1. Younger KG et al. (2016) The effect of prostaglandin F2α on reproductive performance in the postpartum dairy cow and the mechanism behind its benefits. MS Thesis, Oregon State University.

Products used and cited: Nori Bovine IL-1β and IL-6/TNF-α ELISA kits.

  1. Aziza AE et al. (2016) Effect of supplementation of broiler diets with fish oil and linseed oil on growth performance, cytokines and cecal histopathological changes in broiler chickens infected by Eimeria Tenella. Int J Agri Vet Med 4(4):12-27. Impact factor: NA.

Products used and cited: Nori Chicken IL-1, IL-6 and TNF-alpha ELISA Kits.

  1. Marki N et al. (2017) Safety of intra-articular gold microimplants in horses-a randomized, blinded, controlled experimental study. J Equine Vet Sci. 1-8. DOI: http://dx.doi.org/101016/j.jevs.2017.030.005. Impact factor: 0.882.

Products used and cited: Nori Equine IL-1 beta and TNF-alpha ELISA Kit-Synovial Fluid, GR106041-SF, GR106004-SF.

  1. Lombardi P et al. (2017) The association of aloe and beta-carotene supplementation improve oxidization stress and inflammatory state in pregnant buffalo cow. Buffalo Bulletin, 36(3):491-503. Impact factor: 0.104.

Products used and cited: Nori Bovine IL-1b, IL-10, TNFa and MCP-1 ELISA Kits.

  1. Aziza A and Awadin W (2018) Impact of dietary supplementation of whole flaxseed and flaxseed meal to infected broiler chickens with emeria tenella. Asian J Animal Vet Advances doi:10.3923/ajava.2018.166.174. Impact factor: 0.869.

Products used and cited: Nori Chicken IL-1B, IL-6 and TNFa ELISA Kits.

  1. Leander, AAK (2017) A comparative analysis of equine mesenchymal cells and dermal fibroblasts. The University of Waikato, Master of Philosophy Thesis.

Products used and cited: Nori Equine TNF-alpha, IL-1 beta, IL-6, TGF-beta 1, IL-10 and PGE2 ELISA Kits.

  1. Valentine D et al. (2018) Assessment of the effects of diet and physical rehabilitation on radiographic findings and markers of synovial inflammation in dogs following tibial plateau leveling osteotomy. JAMVA, 252(6):701-709. Impact factor: 1.501.

Product used and cited: Nori Canine IL-1b ELISA Kit-Synovial Fluid

  1. Wu Y et al. (2018) Effects of dietary enterococcus faecium NCIMB11811 supplementation on growth performance and cellular and humoral immune responses in broiler chickens. Poultry Science http://dx.doi.org/10.3382/ps/pey368. Impact factor: 2.35.

Products used and cited: Nori Chicken IL-1b, IL-2, IL-4, IL-6, IL-10, IFNg, TNFa ELISA Kits.

  1. Coppelman ZB (2017) The use of biomarkers to determine the severity osteoarthritis in the tarsus of an older horse population. Master of Science Thesis, University of Minnesota.

Products used and cited: Nori Equine IL-1β, IL-6, IL-8, IL-10 and TNFα ELISA Kits.

  1. Tabynov K et al. (2014) Safety and immunogenicity of a novel cold-adapted modified-live equine influenza virus vaccine. Australian Vet J 92(11):450-7. Impact factor: 0.887.

Products used and cited: Nori Equine IL-1β, IL-6, IL-8, IL-10 and TNFα ELISA Kits.

  1. Mastellone V et al. (2014) Effects on oxidative status and metabolism of curcuma longa alone or associated with krill oil and ribes nigrum in healthy dogs. J Nutritional Ecol Food Res 2(4): 348-52. Impact factor: 0.6.

Products used and cited: Nori Canine IL-1, IL-10 and TNFα ELISA Kits.

  1. Hussein H et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro. Comparative Immunology, Microbiology and Infectious Diseases 45: 40-7. Impact factor: 2.05.

Products used and cited: Nori Equine IL-1β, IL-6 and TNFα ELISA Kits.

  1. Sureshkumar S et al. (2019) Oral administration of lactobacillus reuteri expressing a 3D8 single-chain variable fragment (scFv) enhances chicken growth and conserves immune homeostasis. 3 Biotech 9:282. Impact factor: 1.497.

Products used and cited: Nori Chicken IL-1β, IL-4, IL-6, IL-8, IFNγ, IGF1, and TNFα ELISA Kits.

  1. Atteya MA et al. (2019) Evaluation of the prevalence and oxidative status in sheep infected with sarcoptic scabiei in Ismailia Governorate, Egypt. Egyptian Vet Med Society of Parasitology J 15:114-29. Impact factor:

Products used and cited: Nori Sheep IL-1 beta ELISA Kits (GR116002).

  1. Sureshkumar S et al. (2020) Administration of L Salivarius expressing 3D8 scFv as a feed additive improved the growth performance, immune homeostasis, and gut microbiota of chickens. Animal Sc J DOI:10.1111/asj.13399. Impact factor: 1.714.

Products and cited: Nori Chicken IL-1 beta, IL-6, IL-8, IL-4, IFN gamma, IGF1 and TNF alpha ELISA Kits (GR106294, GR106334, GR114139, GR106340, GR114082, GR106410, GR114119).

  1. EI-Ashker, et al. (2020) L-carnitine ameliorates immune-metabolic response and improves antioxidant status in goats with pregnancy toxemia. Veterinarski Archiveorg/10.24099/vet.archiv.0706. Impact factor: 0.492.

Products used and cited: Nori Sheep IL-1b and IL-10 ELISA Kits.

  1. Perinelli DR et al. (2020) Rheelogical properties and growth factors content of palate-rich plasma: relevance in veterinary biomedical treatments. Biomedicines 8:429. Impact factor: 3.6.

Products used and cited: Nori Sheep IL-1b and IL-10 ELISA Kits.

  1. Sureshkumar S. et al. (2020) Inclusion of Lactobacsllus salivarius strain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken. Archives of Microbiologyorg/10.1007/s00203-020-02088-3. Impact factor: 1.607.

Products used and cited: Nori Chicken IL-4, IL-6, IL-8, IL-1b, TNFa, IFNg and IGF1 ELISA Kits.

  1. Song B et al. (2021) Effects of age on immune function in broiler chickens. J Animal Sci Biotech 12:42. Impact factor: 4.167.

Products used and cited: Nori Chicken IL-1β, IL-2, IL-4, IL-10 and IFNγ ELISA Kits.

  1. Uribe-Diaz S et al. (2021) Folic acid enhances proinflammatory and antiviral molecular pathways in chicken B-lymphocutes infected with a mild infectious bursal disease virus. British Poultry Sciorg/10.1080/00071668.2021.1958298. Impact factor: 2.095

Products used and cited: Nori Chicken IL-1B and IFNB ELISA Kits.

  1. Eldin H et al. (2021) Clinicopathological and immunological studies on brucellosis. Benha Vet Med J 40:36-39.

Products used and cited: Nori Bovine IL-1B and IL-10 ELISA Kits

  1. Tucker L et al. (2021) Targeting soluble epoxide hydrolase and cyclooxygenases enhance joint pain control, stimulate collagen synthesis, and protect chondrocytes from cytokine-induced apoptosis. Frontier in Vet Sci doi: 10.3389/fvets.2021.685824. Impact factor: 3.12

Products used and cited: Nori Equine IL-1B, IL-6 and TNFA ELISA Kits

  1. Sureshkumar S et al. (2022) Preliminary Study to Investigate the Effect of Lactobacillus Reuteri Administration on Growth Performance, Immunological, Gut Microbiome and Intestinal Mucosa of Chicken. Brazilian J Poult Sci 24(4):001-006. Impact factor: 0.9.

http://dx.doi.org/10.1590/1806-9061-2022-1640.

Products used and cited: Nori Chicken TNFa, IL-4, IL-6, IL-8, IL-1B, IFNg, IGF1 ELISA Kit.

  1. Uribe-Diaz S et al. (2021) Folic acid enhances proinflammatory and antiviral molecular pathways in chicken B-lymphocytes infected with a mild infectious bursal disease virus. British Poult Sci https://doi.org/10.1080/00071668.2021.1958298. Impact factor: 2.1.

Products used and cited: Nori Chicken IL-1B, IFNb ELISA Kit.

  1. Adamic N et al. (2022) Effect of intrabronchial administration of autologous adipose‑derived mesenchymal stem cells on severe equine asthma. Stem Cell Res & Therapy 13:23. https://doi.org/10.1186/s13287-022-02704-7. Impact factor: 7.9

Products used and cited: Nori Equine IL-1B, IL-4, IL-8, IL-17, TNFa, IFNg ELISA Kit.

  1. Vineet Kumar Mishra et al. (2025) PCFT-Independent Cellular Uptake of Cyclic Cell-Penetrating Peptide-Conjugated Folic Acid. ChemBioChem 26:e202500242. https://doi.org/10.1002/cbic.202500242. Impact factor: 3.0

Products used and cited: Nori Chicken IL-1B, IFNb ELISA Kit; mouse IL-1B, IL-10, TNFa ELISA kit.

  1. Merwid-Lad A et al. (2025) 1,3,4-Oxadiazole Derivatives of Pyrrolo[3,4-d]pyridazinone Alleviate TNBS-Induced Colitis and Exhibit No Significant Testicular Toxicity. Pharmaceuticals 18:546. https://doi.org/10.3390/ph18040546. Impact factor: 4.9

Products used and cited: Nori Rat IL-1B, MMP-9 ELISA Kit.

  1. Song B et al. (2022) Comparison and Correlation Analysis of Immune Function and Gut Microbiota of Broiler Chickens Raised in Double-Layer Cages and Litter Floor Pens. Microbiol Spectrum 10(4). https://doi.org/10.1128/spectrum.00045-22. Impact factor: 8

Products used and cited: Nori Chicken IL-1B, IL-4, IL-10, IFNg ELISA Kit.

  1. Sayed-Elahi RMH et al. (2022) Controlling immunomodulation effects of deoxynivalenol mycotoxins by nanozinc oxide and probiotic in broiler chickens. J World Poult Res 12(3):133-141. Impact factor: 4.0

Products used and cited: Nori Chicken IL-1B, TNFa ELISA Kit

  1. Song B et al. (2022) Effects of Dietary Astragalus Polysaccharide Supplementation on the Th17/Treg Balance and the Gut Microbiota of Broiler Chickens Challenged With Necrotic Enteritis. frontiers in Immunol https://doi.org/10.3389/fimmu.2022.781934. Impact factor: 6.8

Products used and cited: Nori Chicken IL-1B, IL-4, IL-10, IL-17, IFNg ELISA Kit.

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