Nori Sheep IL-6 ELISA MultiSet Kit
$659.00
This ELISA kit is for quantification of IL-6 in sheep. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL6 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-6: Interleukin 6, IL6
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Sheep IL-6 ELISA MultiSet Kit Summary
Alternative names for IL-6: Interleukon 6, IL6,
Materials provided for 10-11 of 96-well plates
| Description | Quantity | Description | Quantity | Description | Quantity |
| Capture Antibody | 1 | Detection Antibody | 1 | Standard
|
3 |
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P29455 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 15 pg/mL |
| Detection Range | 78-5000 pg/mL |
| Specificity | Sheep IL-6 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2.Plate preparation.
3. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
4. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
5. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
6. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
7. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine and is produced by T cells, macrophages, fibroblasts, osteoblasts, endothelial and other cells (1,2,3). IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 is one of the most important mediators of fever and of the acute phase response. In the muscle and fatty tissue, IL-6 stimulates energy mobilization that leads to increased body temperature. IL-6 can be secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPS). IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα that through association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/STAT cascade and the SHP2/Erk Map kinase cascade (1,4,5). IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated (4). The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL6Rα (4). IL-6 is relevant to many disease processes such as diabetes (6), atherosclerosis (7), depression (8), Alzheimer’s Disease (9), systemic lupus erythematosus (10), prostate cancer (11), breast cancer (12), and rheumatoid arthritis (13).
References
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- Febbraio MA and Pedersen BK (2005). Exerc Sport Sci Rev 33 (3): 114–9.
- Jones, S.A. (2005) J Immunol 175, 3463-8.
- Jenkins, B.J. et al. (2004) Mol Cell Biol 24, 1453-63.
- Kristiansen OP and Mandrup-Poulsen T (2005). Diabetes 54 Suppl 2: S114–24.
- Dubiński A and Zdrojewicz Z (2007). Pol. Merkur. Lekarski 22 (130): 291–4.
- Dowlati Y, et al (2010). Biological Psychiatry 67 (5): 446–457.
- Swardfager W, et al (2010). Biological Psychiatry 68 (10): 930–941.
- Tackey E, et al (2004). Lupus 13 (5): 339–43.
- Smith PC, et al (2001). Cytokine Growth Factor Rev. 12 (1): 33–40.
- Hong, D.S. et al. (2007) Cancer 110, 1911-28.
- Nishimoto N (2006). Curr Opin Rheumatol 18 (3): 277–81
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