Nori Rabbit IL-10 ELISA Kit - Image 1
Nori Rabbit IL-10 ELISA Kit - Image 2

Nori Rabbit IL-10 ELISA Kit

Nori Rabbit IL-4 ELISA Kit
Nori Rabbit TNFa ELISA Kit
0 out of 5
( There are no reviews yet. )

Price range: $508.00 through $916.00

DataSheet   

This ELISA kit is for quantification of IL-10 in rabbit. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-10 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for IL-10: IL10, interleukin 10, cytokine synthesis inhibitory factor (CSIF),

This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR144180 Categories: , Tag:

Description

Nori Rabbit IL-10 ELISA Kit Summary

Alternative names for IL-10: IL10, interleukin 10, cytokine synthesis inhibitory factor (CSIF),

 

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number Q9TSJ4
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity 5 pg/mL
Detection Range 25-1600 pg/mL
Specificity  Rabbit IL-10
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

IL-10, also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine that is produced by T cells, NK cells, mast cells and macrophages (1,2,3). It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3 (1,4). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS-1 (1,4). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (5,6). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (7).

References

  1. Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
  2. Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
  3. Grimbaldeston MA, et al (2007). Nat. Immunol. 8 (10): 1095–104.
  4. O’Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
  5. Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
  6. Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
  7. Von Stebut, E. (2000) Eur J Dermatol 17, 115-22.

 

 

 

Product Citations

Nori® IL-10 ELISA Kit Citations

Since 2014, updated on 5-Apr-26

  1. Kaylee Shevon Kohlhaas (2013) Effects of resveratrol supplementation on metabolic health and reproductive performance in obese mares on pasture. MS thesis, Virginia Tech Univ. Product used and cited: Nori Equine TNFa and IL-10 ELISA kits
  2. El-Ashker M et al. (2013) Traumatic reticuloperitonitis in water buffalo (Bubalus bubalis): clinical finding and the associated inflammatory response. J Vet Med ID:808656, 1-6. Impact factor: 0.70. Product used and cited: Nori Bovine IL-10 ELISA kit
  3. Norton J. et al. (2013) Effect of clenbuterol on tracheal mucociliary transport in horses undergoing simulated long-distance transportation. Vet Intern Med 27(6): 1523-7. Impact factor: 2.0. Product used and cited: Nori Equine IL-2, IL-10, TNFα ELISA kits
  4. El-Atahker et al. (2014) The use of inflammatory marker as a prognostic aid for traumatic reticuloperitonitis in water buffalo (Bubalus bubalis). Veterinarni Medicina 59(5):239-246. Impact factor: 0.489. Product used and cited: Nori Bovine IL-10 ELISA kit.
  5. El-Ashker M et al. (2015) Vitamin C ameliorates gentamicin-induced acute kidney injury in equines: an experimental study. Equine Vet Sci. 35:1-6. Impact factor: 0.29. Products used and cited: Nori Equine IL-β, IL-6, IL-10 and IFNg ELISA kits.
  6. Kopp L et al. (2015) The effects of dental pulp derived stem cells on the interleukin response in equine osteoarthritis. The Ohio State University Honors Theses. Products used and cited: Nori Equine IL-1β, IL-1RA, IL-6 and IL-10 ELISA kits.
  7. El-Ashker M et al. (2014) Gentamicin-induced acute kidney injury in equines is associated with marked acute phase response: an experimental study on donkey (Equus asinus). J Vet Sci Med Diagn 4:2. Impact factor: 0.65. Products used and cited: Nori Equine IL-1β, IL-6, IFN-g, IL-10, SAA, Haptoglobin, CRP and SA ELISA kits.
  8. Kopp L et al. (2015) Synovial fluid parameters from osteoarthritic equine joints treated with intra-articulara equine stem cells or control solution. Advances in Veterinary Medicine Research, 2 April 2015, Books of Abstracts, The Ohio State University Press, p23:CR-15. Products used and cited: Nori Equine IL-1β, IL-1RA, IL-6 and IL-10 ELISA kits.
  9. Da Silva et al. (2014) Horse seropositive for neospora spp.: Immunoglobulin, cytokines, and c-reactive protein levels. J Equine Vet Sci. 34(10):1240-143. Impact factor: 0.882. Products used and cited: Nori Equine TNF-α, IFNg, IL-1β, IL-1RA, IL-4, IL-6 and IL-10 ELISA kits.
  10. Do Carmo GM (2015) Immunological response and markers of cell damage in seropositive horses for Toxoplasma gondii. Comparative Immunology, Microbiology and Infectious Diseases. 38:9-13. Impact factor: 1.875. Products used and cited: Nori Equine IL-1β, IL-4, IL-6, IL-10, IFNg and TNF-α ELISA Kits
  11. Abdelrazek HMA et al. (2015) Effect of probiotic strains mixture administration on serum interleukin concentrations, lymphocyte proliferation and DNA damage in rams. J Animal & Feed Sciences 24: 302-307. Impact factor: 1.024. Product used and cited: Nori Sheep IL-10 ELISA kit, Cat. 106454-2.
  12. El-Ashker MR et al. (2017) The influence of experimentally induced endotoxaemia on clinical variables and markers of systemic inflammation in donkeys (Equus asinus). Veterinarni Medicina 62(3):117-124. Impact factor: 0.877. Product used and cited: Nori Equine IL-10, IL-12 and TNF-alpha ELISA Kits.
  13. Baqucro MM et al. (2017) Modulation of effector functions of bovine mononuclear phagocytes by T lymphocytes during in vitro Mycobacterium avium subspecies paratuberculosis infection. PhD Thesis, The University of Guelph. Product used and cited: Nori Bovine IL-10 ELISA Kit GR106295
  14. Canciello A et al. (2017) Progesterone prevents epithelialmesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties. Nature, 7:3761. DOI:10.1038/s41598-017-03908-1. Impact factor: 40.137. Products used and cited: Nori Sheep IL-4, IL-10 and TGFb3 ELISA Kits, GR106451, GR106454, GR106138.
  15. Marki N et al. (2017) Safety of intra-articular gold microimplants in horses-a randomized,
  16. Lombardi P et al. (2017) The association of aloe and beta-carotene supplementation improve oxidization stress and inflammatory state in pregnant buffalo cow. Buffalo Bulletin, 36(3):491-503. Impact factor: 0.104. Products used and cited: Nori Bovine IL-1b, IL-10, TNFa and MCP-1 ELISA Kits.
  17. Sun Y et al. (2017) Oral administration of saccharomyces boulardii alters duodenal morphology, enzymatic activity and cytokine production response in broiler chickens. Animal Sci J, 88:1204-1211. Impact factor: 1.325. Products used and cited: Nori Chicken IL-6, TNFa, IL-10 and TGFb ELISA kits.
  18. Leander, AAK (2017) A comparative analysis of equine mesenchymal cells and dermal fibroblasts. The University of Waikato, Master of Philosophy Thesis. Products used and cited: Nori Equine TNF-alpha, IL-1 beta, IL-6, TGF-beta 1, IL-10 and PGE2 ELISA Kits.
  19. Pi Y et al. (2019) Rubber seed oil and flaxseed oil supplementation on serum fatty acid profile, oxidative-stability of serum and milk and immune function of dairy cows. Asian-Australian J Animal Sci. 32(9):1363-1372. Impact factor: 0.971. Products used and cited: Nori Bovine IL-2, IL-4, IL-6, IL-10, IFNg, TNFa and PGE2 ELISA Kits.
  20. Zheng G et al. (2019) Biologically active 1,25-dihydroxyvitamin D3 protects against experimental sepsis by negatively regulating the toll-like receptor 4/myeloid differentiation primarily response gene 88/Toll-IL-1 resistance-domain-containing adapter-inducing interferon-β signaling pathway. Intl J Molecular Med. 44(3):1151-61. Impact factor: 2.928. Products used and cited: Nori Human IL-6, IL-10 and TNFα ELISA Kits (GR106328-2, GR106292-2, GR106397-2).
  21. Mansour MM et al. (2018) The relationship between blood cytokines concentrations and fertility aspects during natural mastitis in Buffaloes. J Agri Vet Sci 11(2): 80-94. Impact factor: 1.72. Products used and cited: Nori Bovine IL-10 ELISA Kit.
  22. Musco M et al. (2019) Effects of a nutritional supplement in dogs affected by osteoarthritis. Vet Med Sci 5(3): 325-35. Impact factor: 0.943. Products used and cited: Nori Canine IL-6, IL-10 and TNFα ELISA Kits.
  23. Coppelman ZB (2017) The use of biomarkers to determine the severity osteoarthritis in the tarsus of an older horse population. Master of Science Thesis, University of Minnesota. Products used and cited: Nori Equine IL-1β, IL-6, IL-8, IL-10 and TNFα ELISA Kits.
  24. Tabynov K et al. (2014) Safety and immunogenicity of a novel cold-adapted modified-live equine influenza virus vaccine. Australian Vet J 92(11):450-7. Impact factor: 0.887. Products used and cited: Nori Equine IL-1β, IL-6, IL-8, IL-10 and TNFα ELISA Kits.
  25. Mastellone V et al. (2014) Effects on oxidative status and metabolism of curcuma longa alone or associated with krill oil and ribes nigrum in healthy dogs. J Nutritional Ecol Food Res 2(4): 348-52. Impact factor: 0.6. Products used and cited: Nori Canine IL-1, IL-10 and TNFα ELISA Kits.
  26. Baquero MM, Plattner BL (2016) Bovine WC1+ rδ T lymphocytes modify monocyte-derived macrophage responses during early Mycobacterium avium subspecies paratuberculosis infection. Vet Immuno Immunopathol 170: 65-72. Impact factor: 1.846. Products used and cited: Nori Bovine IL-10 ELISA Kit.
  27. Marteles D et al. (2019) Effects of allergen-specific immunotherapy on peripheral blood regulatory T cells and serum concentrations of cytokines and immunoglobulins in horses with allergic dermatitis. Intl Immunopharmacol 74:105674. Impact factor: 1.846. Products used and cited: Nori Equine TGFβ1, IL-10, and IgE ELISA Kits.
  28. Greco L et al. (2020) Characterization of endocannabinoid system and interleukin profiles in ovine AEC: cannabinoid receptors type-1 and type-2 as key effectors of proinflammatory response. Cells 9, 1008, doi:10:3390/cells9041008. Impact factor: 36.216. Products used and cited: Nori Sheep IL-4, IL-10 and IL-12 ELISA Kits (GR106451, GR106454, GR106407).
  29. Canciello A et al. (2020) Progesterone prolongs viability and anti-inflammatory functions of explanted preterm ovine amniotic membrane. Frontiers in Bioengineering and Biotechnology 8 Article 135. frontiersin.org. Impact factor: 3.52. Products used and cited: Nori Sheep IL-4, IL-10 and TGFb1 ELISA Kits (GR106451, GR106454, GR106127).
  30. Mastellone V et al. (2020) A nutritional supplement (DiLshTM) improves the inflammatory cytokine response, oxidative stress markers and clinical signs in dog naturally infected by Leishmania infantum. Animals 10, 398, doi:10.3390/ani10060938. Impact factor: 1.98. Products used and cited: Nori Canine IL-6, IL-10, Leptin and TNF alpha ELISA Kits (GR106143, GR106165, GR115311, GR106171).
  31. EI-Ashker, et al. (2020) L-carnitine ameliorates immune-metabolic response and improves antioxidant status in goats with pregnancy toxemia. Veterinarski Archiveorg/10.24099/vet.archiv.0706. Impact factor: 0.492. Products used and cited: Nori Sheep IL-1b and IL-10 ELISA Kits.
  32. Perinelli DR et al. (2020) Rheelogical properties and growth factors content of palate-rich plasma: relevance in veterinary biomedical treatments. Biomedicines 8:429. Impact factor: 3.6. Products used and cited: Nori Sheep IL-1b and IL-10 ELISA Kits.
  33. Song B et al. (2021) Effects of age on immune function in broiler chickens. J Animal Sci Biotech 12:42. Impact factor: 4.167. Products used and cited: Nori Chicken IL-1β, IL-2, IL-4, IL-10 and IFNγ ELISA Kits.
  34. Eldin H et al. (2021) Clinicopathological and immunological studies on brucellosis. Benha Vet Med J 40:36-39. Products used and cited: Nori Bovine IL-1B and IL-10 ELISA Kits
  35. Gellin GL (2021) An assessment of the carrier state and a novel marker of leptospira and abortion in central Kentucky horses. University of Kentucky UKnoledgeorg/10.13023/etd.2021.371. Products used and cited: Nori Equine IL-10 ELISA Kit
  36. Migda A et al. (2022) Effectiveness of immunization with multi‑component bacterial immunomodulator in foals at 35th day of life. Nature Portfolio 12:15795. https://doi.org/10.10381/s41598-022-17532-1. Impact factor: 15.7. Products used and cited: Nori Equine IgG2, IgM, IL-6, IL-10 ELISA Kits.
  37. Abd EI-Nasser Khattab et al. (2023) Anti‑inflammatory and Immunomodulatory Potency of Selenium‑Enriched Probiotic Mutants in Mice with Induced Ulcerative Colitis. Biological Trace Element Res 201:353-367. Impact factor: 3.8. Products used and cited: Nori Mouse IgG, IgM, IL-2, IL-6, IL-10, TNFa ELISA Kit.
  38. Vineet Kumar Mishra et al. (2025) PCFT-Independent Cellular Uptake of Cyclic Cell-Penetrating Peptide-Conjugated Folic Acid. ChemBioChem 26:e202500242. https://doi.org/10.1002/cbic.202500242. Impact factor: 3.0. Products used and cited: Nori Chicken IL-1B, IFNb ELISA Kit; mouse IL-1B, IL-10, TNFa ELISA kit.
  39. Song B et al. (2022) Comparison and Correlation Analysis of Immune Function and Gut Microbiota of Broiler Chickens Raised in Double-Layer Cages and Litter Floor Pens. Microbiol Spectrum 10(4). https://doi.org/10.1128/spectrum.00045-22. Impact factor: 8. Products used and cited: Nori Chicken IL-1B, IL-4, IL-10, IFNg ELISA Kit.
  40. Song B et al. (2022) Effects of Dietary Astragalus Polysaccharide Supplementation on the Th17/Treg Balance and the Gut Microbiota of Broiler Chickens Challenged With Necrotic Enteritis. frontiers in Immunol https://doi.org/10.3389/fimmu.2022.781934. Impact factor: 6.8. Products used and cited: Nori Chicken IL-1B, IL-4, IL-10, IL-17, IFNg ELISA Kit.

 

 

 

Reviews

There are no reviews yet.

Be the first to review “Nori Rabbit IL-10 ELISA Kit”