Nori Bovine GLUT1 ELISA Kit

$461.00$832.00

DataSheet   

This ELISA kit is for quantification of GLUT1 in bovine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for GLUT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLUT1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for GLUT1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of GLUT1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for GLUT1: Glucose transporter 1 (GLUT1), solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1),

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR112271 Categories: ,
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Description

Nori Bovine GLUT1 ELISA Kit Summary

Alternative names for GLUT1: Glucose transporter 1 (GLUT1), solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1),

Alternative names for bovine: cow, cattle, bull

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number P27674
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity 30 pg/mL
Detection Range 0.156-10 ng/mL
Specificity Bovine GLUT1
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

Glucose transporter 1 (or GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein that in humans is encoded by the SLC2A1 gene.[2] GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells.[3] Energy-yielding metabolism in erythrocytes depends on a constant supply of glucose from the blood plasma, where the glucose concentration is maintained at about 5mM. Glucose enters the erythrocyte by facilitated diffusion via a specific glucose transporter, at a rate about 50,000 times greater than uncatalyzed transmembrane diffusion. GLUT1 is responsible for the low-level of basal glucose uptake required to sustain respiration in all cells. Expression levels of GLUT1 in cell membranes are increased by reduced glucose levels and decreased by increased glucose levels. GLUT1 is also a major receptor for uptake of Vitamin C as well as glucose, especially in non-vitamin C producing mammals as part of an adaptation to compensate by participating in a Vitamin C recycling process. In mammals that do produce Vitamin C, GLUT4 is often expressed instead of GLUT1.[5] It is widely distributed in fetal tissues. In the adult it is expressed at highest levels in erythrocytes and also in the endothelial cells of barrier tissues such as the blood–brain barrier. GLUT1 behaves as a Michaelis-Menten enzyme and contains 12 membrane-spanning alpha helices, each containing 20 amino acid residues. Mutations in the GLUT1 gene are responsible for GLUT1 deficiency or De Vivo disease, which is a rare autosomal dominant disorder.[6] This disease is characterized by a low cerebrospinal fluid glucose concentration (hypoglycorrhachia), a type of neuroglycopenia, which results from impaired glucose transport across the blood–brain barrier. GLUT1 is also a receptor used by the HTLV virus to gain entry into target cells.[7] Glut1 has also been demonstrated as a powerful histochemical marker for haemangioma of infancy[8]

References

  1. Mueckler M, et al. (1985). Science 229 (4717): 941–5.
  2. Olson AL, Pessin JE (1996). Rev. Nutr. 16: 235–56.
  3. Montel-hagen A, et al. (2008). Cell 132 (6): 1039–1048.
  4. Seidner G, et al. (1998). Genet. 18 (2): 188–91.
  5. Manel N, et al. (2003). Cell 115 (4): 449–59.
  6. North PE, et al. (2000). Pathol. 31 (1): 11–22.

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