Nori Human IL2RB ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of IL2RB in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL2RB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL2RB present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL2RB is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL2RB bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL2RB: Interleukin-2 receptor subunit beta (IL2RB), CD122; IL15RB; P70-75, IL-2RB
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Product Citation ()
- Reviews (0)
Description
Nori Human IL2RB ELISA Kit Summary
Alternative names for IL2RB: Interleukin-2 receptor subunit beta (IL2RB), CD122; IL15RB; P70-75, IL-2RB
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P14784 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 12 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Specificity | Human IL2RB |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin-2 receptor subunit beta (IL2RB) is a protein that in humans is encoded by the IL2RB gene.[1] Also known as CD122; IL15RB; P70-75. The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit (also called CD25) and is not involved in signal transduction. The intermediate affinity form consists of a gamma/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. This protein also forms one of the three subunits of the IL-15 receptor. Activation of the receptor increases proliferation of CD8+ effector T cells.[2] IL2RB has been shown to interact with CISH,[3] HGS,[4] Janus kinase 1,[5][6] and SHC1.[7][8]
References
- Tsudo M, et al. (1989). Proc. Natl. Acad. Sci. U.S.A. 86(6): 1982–6.
- Boyman O, Sprent J (2012). Nat Rev Immunol. 12(3): 180–190.
- Aman MJ, et al. (1999). J. Biol. Chem. 274(42): 30266–72.
- Yamashita Y, et al. (2008). J. Cell Sci. 121(Pt 10): 1727–38.
- Miyazaki T, et al. (1994). Science. 266(5187): 1045–7.
- Russell SM, et al. (1994). Science. 266(5187): 1042–5.
- Delespine-Carmagnat M, et al. (2000). Eur. J. Immunol. 30(1): 59–68.
- Ravichandran KS, Burakoff SJ (1994). J. Biol. Chem. 269(3): 1599–602.
Be the first to review “Nori Human IL2RB ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.