Recombinant Canine PDGF-BB Protein
Price range: $99.00 through $456.00
The recombinant canine PDGF-BB protein is derived from in vitro expression of canine PDGF-BB gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.
Alternative names for PDGF-BB: Platelet-derived growth factor subunit B
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- Product Citations (3)
- Reviews (0)
Description
Genorise Recombinant Canine PDGF-BB Protein Summary
Alternative names for PDGF-BB: Platelet-derived growth factor subunit B
Alternative name for canine: dog
Product Specifications
| Purity | > 96%, by SDSPAGE under reducing conditions and visualized by silver stain. |
| Endotoxin Level | < 0.1 EU per 1 μg of the protein by the LAL method. |
| Activity | Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol 109:749. The ED50 for this effect is typically 1-5 ng/mL. |
| Source | E. coli derived canine PDGF-BB. |
| Accession # | Q6Q717 |
| N-Terminal Sequence Analysis | Ser |
| Amino Acid Sequence | Ser82-Thr190 |
| Predicted Molecular Mass | 12.3 kDa |
| SDS-PAGE | 12.3 kDa, reducing conditions |
Background:
Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene (1,2). The protein is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
Though it is synthesized, stored and released by platelets upon activation, it is produced by a plethora of cells including smooth muscle cells, activated macrophages, and endothelial cells. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal (3).
Reference
- Ratner L, et al. (Sep 1985). Nucleic Acids Res 13 (14): 5007–18.
- Clements JM, et al. (Jan 1992). EMBO J 10 (13): 4113–20.
- Hoch RV, Soriano P (2003). Development 130 (20): 4769–4784.
Product Citations
- Birdwhistell K et al. (2017) Validation of commercial ELISAs for quantifying anabolic growth factors and cytokines in canine ACD-A anticoagulated plasma. J Vet Diagnostic Investigation, 1-5, DOI:10.1177. Impact factor: 1.30. Product used and cited: Genorise Recombinant Canine PDGF-BB.
- Franklin SP et al. (2017) Influence of cellular components and exogenous activation on growth factors and cytokine concentrations in canine plate-rich plasma. Frontiers in Veterinary Sciences doi:10.3389. Impact factor: NA. Product used and cited: Genorise recombinant canine PDGF-BB
- Ludwig HC et al. (2017) Use of cyclooxygenase-2 inhibitor does not inhibit platelet activation or growth factor release from platelet-rich plasma. The American Journal of Sports Medicine 45(14):3351-3357. Impact factor: 5.673. Product used and cited: Genorise Recombinant Canine PDGF-BB.
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