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Recombinant Porcine IGF-1 Protein

Placeholder Recombinant Monkey CD98 Protein
Nori Porcine A2M ELISA Kit
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DataSheet   

The recombinant porcine IGF-1 protein is derived from in vitro expression of porcine IGF-1 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for IGF-1: Insulin-like growth factor 1

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Genorise Recombinant Porcine IGF-1 Protein Summary

Alternative names for IGF-1: Insulin-like growth factor 1

 

Product Specifications

Purity > 97%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 1.0 EU per 1 μg of the protein by the LAL method.
Activity Measured in a serum-free cell proliferation assay using MCF7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.

The ED50 for this effect is typically 0.4-1.6 ng/ml.

Background: 

Insulin-like growth factor 1 (IGF-1) is a hormone similar in molecular structure to insulin.[1] It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure.[2] IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7,649 daltons.[3] IGF-1 is produced throughout life primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone (GH) and can be retarded by under-nutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. IGFBP-1 is regulated by insulin. The highest rates of IGF-1 production occur during the pubertal growth spurt. The lowest levels occur in infancy and old age. Its primary action is mediated by binding to its specific receptor, the insulin-like growth factor 1 receptor (IGF1R), which is present on many cell types in many tissues. IGF-1 is a primary mediator of the effects of growth hormone (GH). Patients with severe primary insulin-like growth factor-1 deficiency (IGFD) may be treated with either IGF-1 alone or in combination with IGFBP-3.[4] Mecasermin (brand name Increlex) is a synthetic analog of IGF-1 which is approved for the treatment of growth failure.[4] IGF-1 has been manufactured recombinantly on a large scale using both yeast and E. coli.

References

  1. Jansen M, et al. (1983). Nature 306 (5943): 609–11.
  2. Keating G (2008). “Mecasermin”. BioDrugs 22 (3): 177–88.
  3. Rinderknecht E, Humbel R (1978). J Biol Chem 253 (8): 2769–2776.
  4. Rosenbloom A (2007). Opin. Pediatr. 19 (4): 458–64.

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