Nori Rat Tryptase alpha/beta-1 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of Tryptase alpha/beta-1 in rat. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for TPSAB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSAB1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for TPSAB1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of TPSAB1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for Tryptase alpha/beta-1: TPSAB1, tryptase-1, TPS1, tryptase alpha 1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Documents
- Product Citations (0)
- Reviews (0)
Description
Nori Rat Tryptase alpha/beta-1 ELISA Kit Summary
Alternative names for tryptase alpha/beta-1: TPSAB1, tryptase-1, TPS1, tryptase alpha-1,
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P27435 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 0.156-10 ng/mL |
| Specificity | Rat Tryptase alpha/beta-1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Tryptase alpha-1 and beta-1 are an enzymes that are encoded by the same TPSAB1 genes.[1][2] Beta tryptases appear to be the main isoenzymes expressed in mast cells while alpha tryptase is predominately expressed in basophils.[2] Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. It may play a role in regulation of the mRNA stability. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. Tryptase alpha-1 is elevated in those with systemic mastocytosis.[3] Tryptase beta-1 shows fibrinogenolytic activity [4] and is a mitogen for epithelial cell and stimulates IL-8 and intercellular adhesion molecule-1 productions.[5] Tryptase beta-1 stimulates the synthesis of type I collagen in human lung fibroblasts.[6]
References
- Miller JS, et al. (1990). J Clin Invest. 86 (3): 864–70.
- Pallaoro M, et al. (1999). J Biol Chem. 274 (6): 3355–62.
- Schwartz LB, et al. (1996). J. Clin. Invest. 96 (6): 2702–10.
- Schwartz LB, (1985). J. Immunol. 135 (4): 2762–7.
- Cairns JA, Walls AF (1996). J. Immunol. 156 (1): 275–83.
- Cairns JA, Walls AF (1997) J. Clin. Invest. 99 (6): 1313–21.
Product Citations
Be the first to review “Nori Rat Tryptase alpha/beta-1 ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.