Nori Bovine SFRP5 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of SFRP5 in bovine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for SFRP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP5 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for SFRP5 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of SFRP5 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for SFRP5: Secreted frizzled-related protein 5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3,
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Documents
- Product Citation (0)
- Reviews (0)
Description
Nori Bovine SFRP5 ELISA Kit Summary
Alternative names for SFRP5: Secreted frizzled-related protein 5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3,
Alternative names for bovine: Cattle, cow, bull, buffalo
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
Q9XSC1 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 2 pg/mL |
| Detection Range | 12.5-800 pg/mL |
| Specificity | Bovine SFRP5 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins.[1,2] SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.[2] Epigenetic inactivation of the Secreted frizzled-related protein-5 (SFRP5) gene in human breast cancer is associated with unfavorable prognosis.[4] SFRP5 is activated via interaction with its receptor.[5]
References
- Melkonyan HS, et al. (1998). Proc Natl Acad Sci U S A 94 (25): 13636–41.
- Chang JT, et al. (1999). Mol. Genet. 8 (4): 575–83.
- Hu E, et al. (1998). Biophys. Res. Commun. 247 (2): 287–93.
- Veeck J, et al. (2008). 29 (5): 991–998.
- Rattner A, et al. (1997). Natl. Acad. Sci. U.S.A. 94 (7): 2859–63.
Be the first to review “Nori Bovine SFRP5 ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.