Nori Human IL-3 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of IL-3 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL3 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL3 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL3 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-3: Interleukin 3, MCGF
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Documents
- Product Citation
- Reviews (0)
Description
Nori Human IL-3 ELISA Kit Summary
Alternative names for IL-3: Interleukin 3, MCGF
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
P08700 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 3 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Specificity | Human IL-3 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin 3 (IL-3) is a protein that is encoded by the IL3 gene[1] and it is called colony-stimulating factor; mast cell growth factor, MULTI-CSF, MCGF; MGC79398, MGC79399. The protein contains 152 amino acids and its molecular weight is 17 kDa and the naturally occurring IL-3 is glycosylated. IL-3 is produced as a monomer by activated T cells, monocytes/macrophages and stroma cells. The major function of IL-3 cytokine is to regulate blood-cell production. It induces proliferation and differentiation of early pluripotent stem cells and committed progenitors.[2] Interleukin 3 acts by binding to the interleukin-3 receptor.[3] IL-3/Receptor complex induces JAK2/STAT5 cell signalisation pathway. It can stimulate transcription factor c‑myc (activation of gene expression) and Ras pathway (suppression of apoptosis). Interleukin 3 has been shown to interact with IL3RA.[4] Interleukin 3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
References
- Yang YC, et al. (1986). Cell. 47 (1): 3–10. doi:1016/0092-8674(86)90360-0. PMID3489530.
- Aglietta, M.; et al. (1996). Stem Cells. 11 (S2): 83–87.
- Tabira T, et al. (1998). Ann. N. Y. Acad. Sci. 840 (1): 107–16.
- Stomski FC, et al. (1996). Mol. Cell. Biol. 16 (6): 3035–46.
Product Citation
Be the first to review “Nori Human IL-3 ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.