Nori Human DPP4 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of DPP4 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for DPP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPP4 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for DPP4 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of DPP4 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for DPP4: Dipeptidyl peptidase-4, adenosine deaminase complexing protein 2, CD26
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Description
Nori Human DPP4 ELISA Kit Summary
Alternative names for DPP4: Dipeptidyl peptidase-4, adenosine deaminase complexing protein 2, CD26
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
P27487 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 5 pg/mL |
| Detection Range | 25-1600 pg/mL |
| Specificity | Human DPP4 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 is a protein that is encoded by the DPP4 gene.[1] DPP4 is related to attractin, FAP, DPP8 and DPP9. It is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP-4 also binds the enzyme adenosine deaminase specifically and with high affinity. The substrates of DPP4 are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1.[2] Furthermore, it appears to work as a suppressor in the development of cancer and tumours.[3][4] Animal studies suggest its pathogenetic role in development of fibrosis of various organs, such as liver and kidney.[5] DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.[6] A new coronavirus related to SARS, named Middle East respiratory syndrome coronavirus, has been found to bind to DPP4. DPP4 is found on the surface of cells in the airways (such as the lungs) and kidneys. Scientists may be able to use this to their advantage by blocking the virus’s entry into the cell.[7]
References
- Kameoka J, et al. (1993). Science 261 (5120): 466–9.
- Barnett A (Nov 2006). International Journal of Clinical Practice 60 (11): 1454–70.
- Masur K, et al. (2006). Regulatory Peptides 137 (3): 147–55.
- Wesley UV, et al. (2005) Cancer Research 65 (4): 1325–34.
- Kaji K, et al. (2014). Journal of Gastroenterology 49 (3): 481–91.
- Havre PA, et al. (2008). Frontiers in Bioscience 13 (13): 1634–45.
- Raj VS, et al. (2013). Nature (ScienceNews) 495 (7440): 251–4.
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