Nori Human CD98 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of CD98 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for CD98 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD98 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for CD98 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of CD98 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for CD98: Cluster of differentiation 98, Amino acid transporter heavy chain SLC3A2, MDU1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human CD98 ELISA Kit Summary
Alternative names for CD98: Cluster of differentiation 98, Amino acid transporter heavy chain SLC3A2, MDU1
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P08195 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 0.156-10 ng/mL |
| Specificity | Human CD98 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
CD98 is a glycoprotein[1][2] that is a heterodimer composed of SLC3A2 and SLC7A5 that forms the large neutral amino acid transporter (LAT1). LAT1 is a heterodimeric membrane transport protein that preferentially transports branched-chain (valine, leucine, isoleucine) and aromatic (tryptophan, tyrosine) amino acids.[3] LAT is highly expressed in brain capillaries (which form the blood–brain barrier) relative to other tissues.[3] A functional LAT1 transporter is composed of two proteins encoded by two distinct genes: 4F2hc/CD98 heavy subunit protein encoded by the SLC3A2 gene and CD98 light subunit protein encoded by the SLC7A5 gene. LAT1 showed a novel fundamental role to support the protein expression of 4F2hc via a chaperone-like function in chorionic trophoblasts.[4] SLC3A2 is upregulated in osteosarcoma and plays a crucial role in tumor growth.[5] Increased CD98 (4F2hc) expression plays an essential role in tumor aggressiveness and metastasis.[6]
References
- Kucharzik T, et al. (2005). Lab. Invest. 85(7): 932–41.
- Lemaître G, et al. (2005). Proteomics. 5(14): 3637–45.
- Boado RJ, et al. (1999). Proc. Natl. Acad. Sci. U.S.A. 96(21): 12079–84.
- Ohgaki R, et al. (2017) Mol. Cell. Biol. 37 (11), e00427-16.
- Zhu B, et al. (2017)J Oncol. Rep. 37 (5), 2575-2582.
- Satoh T, et al. (2017)J Anticancer Res. 37 (2), 631-636.
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