Nori Mouse BMP8a ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of BMP8a in mouse. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for BMP8a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP8a present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for BMP8a is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of BMP8a bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for BMP8a: Bone morphogenetic protein 8A
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Mouse BMP8a ELISA Kit Summary
Alternative names for BMP8a: Bone morphogenetic protein 8A
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P34821 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 5 pg/mL |
| Detection Range | 25-1600 pg/mL |
| Specificity | Mouse BMP8a |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Bone morphogenetic protein 8A (BMP8A) is a protein that is encoded by the BMP8A gene. BMP8A is a polypeptide member of the TGFβ superfamily of proteins and is a disulfide-linked homodimer. It, like other BMPs, is involved in the development of bone and cartilage. BMP8A may be involved in epithelial osteogenesis. It also plays a role in bone homeostasis. Bmp8a, but not Bmp8b, was expressed predominantly in the neonatal human spermatogonia. Although most BMPs induce activation of SMADs 1, 5, and 8 (SMAD1/5/8), but not SMADs 2 and 3 (SMAD2/3), BMP8A induced signaling through both sets of transcription factors.[1] BMP8a is highly expressed in clear cell renal cell carcinoma (ccRCC) suggesting a poor prognosis of ccRCC. BMP8 activates Nrf2 regulated reactive oxygen balance, and by regulating the transcription level of TRIM24, it is involved in the regulation of the Wnt pathway to promote the proliferation, invasion and metastasis of ccRCC and the resistance of As2O3.[2]
References
1. Wu FJ, et al. (2017) BMP8A sustains spermatogenesis by activating both SMAD1/5/8 and SMAD2/3 in spermatogonia. Sci Signal 10 (477).
2. Yu YP, et al. (2020) BMP8A promotes survival and drug resistance via Nrf2/TRIM24 signaling pathway in clear cell renal cell carcinoma. Cancer Sci 111 (5), 1555-1566.
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